KARACHI: Because the pandemic spreads and case statistics enhance by leaps and bounds, Pakistani scientists are getting ready to growing regionally produced diagnostic kits for the novel coronavirus. The kits, to be ready by scientists on the Nationwide Institute of Virology (NIV), with the company of Chinese language researchers, would be the first of its variety to be produced within the nation, declare researchers behind the venture.
The most recent improvement within the venture surfaced when scientists achieved ‘positive control’ of the virus within the first section of experimentation. Since then, preliminary experimentation on the artificial plasmid gene of the coronavirus imported from China has been accomplished whereas orders have been positioned for the import of chemical compounds required for the second stage of the method.
Talking to The Categorical Tribune, Worldwide Middle for Chemical and Biology Sciences (ICCBS) Director Prof Dr Iqbal Chaudhry, confirmed the event of the diagnostic kits on the Nationwide Institute of Virology, a sub-institution beneath ICCBS of the College of Karachi. “COVID-19 is a national threat and we, with the intention of curbing and controlling the disease in the country have begun preparing to produce the kits domestically in Pakistan,” he added.
In response to Dr Mohammad Ammar Athar, a Pakistani scientist engaged on producing the diagnostic kits, they’ve set two targets for the venture. The primary, basic goal is to organize the early diagnostic kits able to diagnosing the bottom limits of the virus, whereas the second goal is to diagnose a number of individuals with a single low-cost package. “The required chemicals are expected to arrive from Canada by next week and it will take us about 18 months from then to prepare the diagnostic kits,” he claimed.
As per Pakistani researchers, the Chinese language imported artificial plasmid gene of the coronavirus has been matched with a designed primer and has confirmed profitable in detecting the virus. “We have obtained a positive control of the virus and changed the primer from a powder form to liquid which is lyophilized to save the biological content,” the researchers knowledgeable.
Thus far, imported coronavirus kits haven’t been completely profitable in detecting the virus regardless of clear signs for the reason that kits aren’t geared up for early detection or low limits of the virus. “However, our primary target for the domestically produced kits is to focus on early detection,” stated Dr Ammar Athar. “Scientifically speaking, the limit of a virus in a human body is called ‘copy’. The kits that have arrived in Pakistan are capable of detecting a viral load 300 copies while we aim to diagnose as low as 50 copies with our own kits. This is a huge target to achieve but if we are able to detect the virus in its early stages before it multiplies, it will make treatment significantly easier,” he added.
As per ICCBS Director Dr Iqbal Chaudhry, sure kits being imported from China and different nations have the capability to check 20 circumstances whereas others can take a look at 48. “However, we are trying to prepare a kit capable of testing over 300 samples and real-time work on PCR technology is being carried out for the preparation of these kits, producing which can cost up to Rs 250,000 to 300, 000 per kit,” Chaudhry additional knowledgeable.
Whereas talking to The Categorical Tribune, Chaudhry additionally assured of full cooperation to the related authorities relating to funding for the venture. “The test is likely to cost between Rs 300 to Rs 500 after the preparation of the diagnostic kits, while the management of a public and private hospital has also been contacted in the regard since samples of a few COVID-19 positive cases are required for the preparation of the kits,” stated the ICCBS director. “Both hospitals have agreed full cooperation but owing to the sensitivity of the matter, the positive test samples required for the preparation of the kit would not be transported but experimented internally at the concerned hospitals,” he concluded.